TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Genotype and/or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| MB98 | aroB tsx malT thi ΔfhuA | 10 |
| MB99 | aroB tsx malT thi ΔfhuA tonB | 2 |
| MB1859 | aroB tsx malT thi ΔfhuACDB tonB | Michael Braun |
| Plasmids | ||
| pHK763 | pT7-6 fhuA wild type | 23 |
| pFE46 | pT7-6 fhuA (Δ502-515 NSEG)a | This study |
| pFE47 | pT7-6 fhuA (Δ394-419 NSEG) | This study |
| pFE48 | pT7-6 fhuA (Δ598-611 NSEGS) | This study |
| pFE61 | pT7-6 fhuA (Δ243-273 NSEGS) | This study |
| pFE62 | pT7-6 fhuA (Δ640-654 NSEGS) | This study |
| pFE69 | pT7-6 fhuA (Δ318-339 NSEGS) | This study |
| pFE73 | pT7-6 fhuA (Δ552-558 NSEG) | This study |
| pFE74 | pT7-6 fhuA (Δ689-701 NSEG A702S) | This study |
| pFE75 | pHSG576 fhuA (Δ243-273 NSEGS) | This study |
| pFE76 | pHSG576 fhuA (Δ318-339 NSEGS) | This study |
| pFE77 | pHSG576 fhuA (Δ394-419 NSEG) | This study |
| pFE78 | pHSG576 fhuA (Δ502-515 NSEG) | This study |
| pFE79 | pHSG576 fhuA (Δ552-558 NSEG) | This study |
| pFE80 | pHSG576 fhuA (Δ598-611 NSEGS) | This study |
| pFE81 | pHSG576 fhuA (Δ640-654 NSEGS) | This study |
| pFE82 | pHSG576 fhuA (Δ689-701 NSEG A702S) | This study |
| pHK570 | pHSG576 fhuA wild type | 20 |
| pT7-6 | ColE1; Ampr; T7 gene 10 promoter | 30 |
| pHSG576 | Cmr; pSC101 origin | 31 |
a
Numbers in parentheses correspond to encoded amino acid residues deleted by the mutation. The indicated peptide was inserted to prevent structural restrictions in β-barrel formation. Plasmids pFE74 and pFE82 carry amino acid change A702S.