TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference(s) and/or source |
|---|---|---|
| Strains | ||
| SL1344 | xyl hisG rpsL | 40 |
| CK5 | SL1344 ΔhisG::Kan | This study |
| CK500 | SL1344 ΔhisG::FRT | This study |
| CK500/pCP20 | CK500/pCP20 | This study |
| SL1344 ΔenvZ::Kan | SL1344 ΔenvZ::Kan | Corrella Detweiler |
| CK101 | SL1344 ΔenvZ::Kan (P22 transduced) | This study |
| CK121 | SL1344 ΔenvZ | This study |
| CK115 | SL1344 ΔackA-pta::Kan | This study |
| CK116 | CK121 ΔackA-pta::Kan | This study |
| CK117 | SL1344 ΔhisG::pCK60 | This study |
| CK118 | SL1344 ΔhisG::pCK61 | This study |
| CK103 | SL1344 ΔhisG::pCK53 | This study |
| CK104 | SL1344 ΔhisG::pCK54 | This study |
| CK119 | SL1344 ΔackA-pta | This study |
| CK120 | SL1344 ΔenvZ ΔackA-pta | This study |
| CK107 | CK121 ΔhisG::pCK53 | This study |
| SL1344 phoP::Tet | SL1344 phoP::Tet | Bruce Stocker |
| CK122 | CK107 phoP::Tet/pTaz1-1 | This study |
| CK123 | CK107 phoP::Tet/pTazT247R | This study |
| CK136 | SL1344 ΔssrAB | This study |
| SL1344 fur/pFMI10 | SL1344 fur-1 zbf::Tn10/pFMI10 | Ferric Fang; this study |
| CJD359/pFMI10 | SL1344 ompR1009::Tn10/pFMI10 | Anthea Lee; this study |
| CK119/pFMI10 | CK119/pFMI10 | This study |
| CK120/pFMI10 | CK120/pFMI10 | This study |
| CK121/pFMI10 | CK121/pFMI10 | This study |
| CK136/pFMI10 | CK136/pFMI10 | This study |
| CK124 | CK121 ΔhisG::pCK60 | This study |
| CK125 | CK121 ΔhisG::pCK61 | This study |
| CK107 | CK121 ΔhisG::pCK53 | This study |
| CK108 | CK121 ΔhisG::pCK54 | This study |
| CK124/pTaz1-1 | CK124/pTaz1-1 | This study |
| CK124/pTazT247R | CK124/pTazT247R | This study |
| CK125/pTaz1-1 | CK125/pTaz1-1 | This study |
| CK125/pTazT247R | CK125/pTazT247R | This study |
| CK107/pTaz1-1 | CK107/pTaz1-1 | This study |
| CK107/pTazT247R | CK107/pTazT247R | This study |
| CK108/pTaz1-1 | CK108/pTaz1-1 | This study |
| CK108/pTazT247R | CK108/pTazT247R | This study |
| Plasmids | ||
| pFMI10 | pFPV25 with ssaG promoter | 45 |
| pCP20 | FLP recombinase expression vector | 7 |
| pCE37 | Promoter lacZ integration vector | 11 |
| pCK52 | pCE37 rrn multiple cloning site | This study |
| pCK60 | pCK52 with ompC promoter | This study |
| pCK61 | pCK52 with ompF promoter | This study |
| pCK53 | pCK52 with ssrA promoter | This study |
| pCK54 | pCK52 with ssaG promoter | This study |
| pTaz1-1 | ColE1 Taz expression vector | 10, 20 |
| pTazT247R | ColE1 Taz (T247R) mutant expression vector | 10, 20 |