FIG 6.

P. falciparum Dyn1 functions in the hemoglobin transport pathway. (A) Representative single-section electron micrograph of IRBC treated with 200 μM Dynasore for 30 min. The arrows indicate unusual tortuous, thin tubes containing hemoglobin that localize to the parasite periphery. Scale bar, 500 nm. (B) Representative 3D reconstruction of IRBC incubated with 200 μM Dynasore for 30 min. Arrowheads point to thin, elongated, hemoglobin-containing tubes that result from Dynasore treatment. (C) Western blot assay showing the expression of FKBP-HA-tagged P. falciparum Dyn1 with or without 0.5 μM Shld1 for 48 h. Whole-cell parasite lysate was probed with an anti-HA antibody. The same amount of protein (50 μg) was loaded into each lane. The third lane is lysate from untransfected parasites. The arrow indicates a size of 100 kDa. (D to G) Immunoelectron micrographs showing intraparasitic localization of FKBP-HA-tagged P. falciparum Dyn1. The arrows indicate gold particle labeling on membranous structures. (E) Black arrow indicating HCs labeling. (F and G) Representative images showing that P. falciparum Dyn1 is absent from cytostomal collars (arrowheads). Scale bar, 250 nm.