FIG 3.

The rate of HXL1 mRNA splicing is delayed in the puf4Δ strain compared to that in the wild type. (A, B) The amount of unspliced and spliced HXL1 mRNA was compared between wild-type and puf4Δ cells. RNA was isolated from wild-type cells or puf4Δ cells during growth at 37°C (A) or treatment with tunicamycin (B). Afterward, the RNA was converted to cDNA and then amplified in a semiquantitative PCR using primers that span the HXL1 splice site and electrophoresed on an agarose gel. Unless indicated otherwise, the numbers above the gels are times (in minutes). The higher-molecular-mass band corresponds to unspliced HXL1 transcript. The lower-molecular-mass band corresponds to spliced HXL1 mRNA. The graphs depict the percentage of spliced HXL1 mRNA. Data are representative of those from at least three biological replicates.