TABLE 1.
Identification of putative PUF elements in UTRs of known HAC1/HXL1 orthologs in fungia
| Species | Strain | Locus identifier | Sequence |
Annotated UTR | |
|---|---|---|---|---|---|
| 5′ UTR | 3′ UTR | ||||
| Cryptococcus neoformans var. grubii | H99 | CNAG_06134 | UGUAACAGUA | UGUACCGAUA | Y |
| Cryptococcus neoformans var. neoformans | Jec21 | CNM01380 | UGUAACAUUA | UGUAUCGAUA | N |
| Cryptococcus neoformans var. neoformans | B-3501A | CNBM1240 | UGUAACAUUA | UGUAUCGAUA | N |
| Cryptococcus gattii | R265 | CNBG_4842.2 | UGUAACAUUA | Y | |
| Cryptococcus gattii | WM276 | CGB_M1590W | N | ||
| Coprinopsis cinerea | Okayama7#130 | CC1G_02249 | UGUAGCAGUA | N | |
| Ustilago maydis | 521 | UM03509.1 | UGUAAAUGUA | N | |
| Yarrowia lipolytica | CLIB122 | XP_500811 | N | ||
| Candida albicans | WO-1 | CAWG_00794.1 | N | ||
| Candida albicans | SC5314 | C1_06130C | N | ||
| Aspergillus fumigatus | H237 | ACJ61678 | Y | ||
| Aspergillus nidulans | FGSC 26 | Q8TFU8 | Y | ||
a
We screened the previously identified HAC1 orthologs (2) for putative PUF elements that match the PUF element in C. neoformans var. grubii HXL1. The putative PUF elements in the HXL1 homolog for each species are listed. Included in the table are both sequences with annotated UTRs (yes [Y]) and sequences without annotated UTRs (no [N]). For genes without annotated UTRs, sequences 500 bp upstream and downstream from the coding sequence were scanned for putative PUF elements. For putative PUF elements with variable regions that do not match the known Puf5p consensus sequence, the nonmatching nucleotide is shown in bold and underlined (18).