FIG 7.

Analysis of host cell cytoplasmic and parasite-associated fractions of P. yoelii 17X- and P. yoelii 17XL-infected RBCs. Immunoblot analysis of host cell cytoplasmic and parasite-associated fractions obtained by saponin treatment of P. yoelii 17X-infected reticulocytes or P. yoelii 17XL-infected mature RBCs was performed. Equal volumes of parasite-associated fractions and a 5-fold-larger volume of the host cell cytoplasmic fraction from P. yoelii 17X- or P. yoelii 17XL-infected RBCs were loaded on the gel. (A to C) The immunoblots were probed with rabbit anti-rPyMSP8 antibodies (A), rabbit anti-P. falciparum aldolase antibodies (B), or rabbit anti-rPyExp2 sera (C). (D) A soluble fraction and a particulate fraction of the initial host cell cytoplasmic fraction from P. yoelii 17X-infected reticulocytes or P. yoelii 17XL-infected mature RBCs were obtained by high-speed centrifugation (20,000 × g; 45 min). The particulate material was resuspended in SDS-PAGE sample buffer in a volume equal to that of the corresponding soluble supernatant. For immunoblot analysis, equal volumes of soluble and particulate fractions from P. yoelii 17X- or P. yoelii 17XL-infected RBCs were loaded on the gel and probed with rabbit anti-rPyExp2 sera.