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Localization of PRO45 in Δpro11 and Δpro22 mutants by SIM. The Δpro11 and Δpro22 mutants were transformed with pEGFP-45 and stained with either DAPI or MitoTracker. (A) SIM shows that EGFP-PRO45 is absent from the nuclear envelope in the Δpro11 mutant but is still associated with mitochondria. (B) Similarly, PRO45 can be found only at the mitochondria and not at the nuclear envelope in the Δpro22 mutant. Bars, 5 μm and 1 μm (insets).
