TABLE 1.
Strains used in this study
| Strain | Relevant genotype and phenotypea | Reference or source |
|---|---|---|
| S91327 | Wild type, fertile | Culture collectionb |
| S84595 | Spore color mutant fus (fus1-1), fertile | Culture collectionb |
| S96888 | Δku70, recipient strain for homologous recombination | 45 |
| N161 | Single-spore isolate, sterile, Δpro45::Hphr | This study |
| R7329 | Single-spore isolate, sterile, Δpro45::Hphr fus | This study |
| N401 | Single-spore isolate, ectopic integration of p45-CTAP into N161, fertile, Δpro45 gpd(p)::pro45::ctap::trpC(t) trpC(p)::nat | This study |
| N506, N508 | Single-spore isolate, ectopic integration of pEGFP-45 into N161, fertile, Δpro45 gpd(p)::egfp::pro45::trpC(t) trpC(p)::nat | This study |
| N861 | Single-spore isolate, ectopic integration of pEGFP-45 and pDsREDKDEL into N161, fertile, Δpro45 gpd(p)::egfp::pro45::trpC(t) trpC(p)::nat gpd(p)::Spro41::DsRED::KDEL::trpC(t) trpC(p)::nat | This study |
| N883 | Single-spore isolate, ectopic integration of pEGFP-45 and pRH2B into N161, fertile, Δpro45 gpd(p)::egfp::pro45::trpC(t) trpC(p)::nat gpd(p)::hh2b::tdTomato::trpC(t) trpC(p)::hph | This study |
| N1032 | Single-spore isolate, ectopic integration of p45ΔFHA-TAP into N161, sterile, Δpro45 gpd(p)::pro45ΔFHA::ctap::trpC(t) trpC(p)::nat | This study |
| N1111 | Single-spore isolate, ectopic integration of p45ΔTM-EGFP into N161, fertile, Δpro45 gpd(p)::pro45ΔTM::egfp::trpC(t) trpC(p)::nat | This study |
| S5.7 | Single-spore isolate, sterile, Δpro11::Hphr | 30 |
| S56 | Single-spore isolate, sterile, Δpro22::Hphr | 30 |
| S106172 | Single-spore isolate, ectopic integration of pDS23 into S91327, fertile, gpd(p)::egfp::trpC(t) trpC(p)::nat | This study |
| S123155 | Single-spore isolate, ectopic integration of pEGFP-45 into S56, sterile, Δpro22 gpd(p)::egfp::pro45::trpC(t) trpC(p)::nat | This study |
| T104.4 | Primary transformant, ectopic integration of p45-EGFP and pHA11 into S91327, fertile, gpd(p)::pro45::egfp::trpC(t) trpC(p)::nat ccg1(p)::HA::pro11 trpC(p)::nat | This study |
| T105.2 | Primary transformant, ectopic integration of p45-EGFP and pFLAGMob3 into S91327, fertile, gpd(p)::pro45::egfp::trpC(t) trpC(p)::nat ccg1(p)::3×FLAG::Smmob3 trpC(p)::hph | This study |
| T1201-A | Primary transformant, ectopic integration of pHA11 into S91327, fertile, ccg1(p)::ha::pro11 trpC(p)::nat | This study |
| T1202-A2 | Primary transformant, ectopic integration of pFLAGMob3 into S91327, fertile, ccg1(p)::3×FLAG::Smmob3 trpC(p)::hph | This study |
| T131-D5 | Primary transformant, ectopic integration of p45-EGFP into S91327, fertile, gpd(p)::pro45::egfp::trpC(t) trpC(p)::nat | This study |
| T133-E6 | Primary transformant, ectopic integration of p45-EGFP and pHA11 into S91327, fertile, gpd(p)::pro45::egfp::trpC(t) trpC(p)::nat ccg1(p)::ha::pro11 trpC(p)::nat | This study |
| T135-B2 | Primary transformant, ectopic integration of pEGFP-45 into S5.7, sterile, Δpro11 gpd(p)::egfp::pro45::trpC(t) trpC(p)::nat | This study |
a
hph, hygromycin resistance gene; nat, nourseothricin resistance gene.
b
Culture collection, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, Bochum, Germany.