TABLE 2.
Plasmids used in this study
| Plasmid | Characteristics | Reference or source |
|---|---|---|
| p45-CTAP | pro45 cDNA fused to a C-terminal TAP tag in pDS22, gpd(p)::pro45::ctap::trpC(t) trpC(p)::nat | This study |
| p45-EGFP | pro45 cDNA in pDS23 gpd(p)::pro45::egfp::trpC(t) trpC(p)::nat | This study |
| p45ΔFHA-TAP | pro45 cDNA (deletion of bp 682–855) fused to a C-terminal TAP tag in pDS22, gpd(p)::pro45ΔFHA::ctap::trpC(t) trpC(p)::nat | This study |
| p45ΔTM-EGFP | pro45 cDNA (deletion of bp 2302–2370) in pDS23, gpd(p)::pro45ΔTM::egfp::trpC(t) trpC(p)::nat | This study |
| pDrivehph | Hygromycin resistance cassette consisting of the hph gene from Escherichia coli and the trpC promoter from Aspergillus nidulans in pDrive | 42 |
| pDS16 | hph gene with 5′ and 3′ flanking regions of SMAC_01224 (pro45) in pRS426 pro45_5′::trpC(p)::hph::pro45_3′ trpC(p)::nat | This study |
| pDS22 | C-terminal TAP tag in pRSnat, gpd(p)::ctap::trpC(t) trpc(p)::nat | Nowrousian, unpublished |
| pDS23 | egfp in pRSnat, gpd(p)::egfp::trpC(t) trpc(p)::nat | 44 |
| pDsREDKDEL | DsRed fused with the N-terminal signal sequence for cotranslational insertion into the ER from pro41 and the C-terminal ER retention signal KDEL, gpd(p)::Spro41::DsRED::KDEL::trpC(t) trpC(p)::nat | 41 |
| pEGFP-45 | pro45 cDNA in pDS23, gpd(p)::egfp::pro45::trpC(t) trpC(p)::nat | This study |
| pFLAGMob3 | Smmob3 cDNA fused to an N-terminal 3× FLAG tag in pRShyg, ccg1(p)::3×FLAG::Smmob3 trpC(p) Hphr | 30 |
| pHA11 | pro11 cDNA fused to an N-terminal HA tag in pRSnat, ccg1(p)::HA::pro11 trpC(p)::nat | 30 |
| pRH2B | Histone H2B fused to TdTomato, gpd(p)::hh2b::tdTomato::trpC(t) trpC(p)::hph | I. Teichert and U. Kück, unpublished data |
| pRS426 | URA3 Hphr | 43 |
| pRSnat | pRS426 derivative, URA3 Natr | 77 |
a
Hphr, hygromycin resistant; Natr, nourseothricin resistant.