Fig. 5.

GluN3 KO mice hindered functional recovery after focal cerebral ischemia. The adhesive removal test was performed to examine the effect of GluN3A on functional recovery before surgery and at 3 and 7 days after focal cerebral ischemia. A: all animals showed increased time to contact after focal cerebral ischemia, indicating impaired function. GluN3 KO mice exhibited significantly hindered sensorimotor activity at 3 days after focal cerebral ischemia. *P < 0.05 vs. WT mice at each time point; n = 6–16 animals in each group. B: time to remove the sticky dot from left paw was significantly increased in GluN3 KO mice compared with WT time at 3 days after focal cerebral ischemia. *P < 0.05 vs. WT mice at each time point; n = 6–16 animals in each group. C and D: travelled distance and velocity were measured using TopScan System analysis. At 3 days after focal cerebral ischemia, GluN3A KO mice exhibited significant less travelled distance and lower velocity than WT mice, indicating impaired locomotor activity. *P < 0.05 vs. WT mice at each time point; n = 4–5 animals in each group. E: cylinder test showed that forelimb activities of mice with focal cerebral ischemia were impaired during the first few days after focal cerebral ischemia. GluN3 KO mice performed significantly worse than WT mice at 3 days after focal cerebral ischemia. *P < 0.05 vs. WT mice at each time point; n = 4–5 animals in each group.