. 2014 Dec 10;6(4):1900–1919. doi: 10.18632/oncotarget.2953

Table 1. Marker profiles of prospective human prostate cancer stem-like cells (PCSCs).

PCSC Marker Profile	Source of PCSCs	Evidence for PCSC Activity in vivo
CD133⁺ or CD24⁺	1° PCa xenograft (SC; TS, IC mice)	Purified cells generate 2° tumors at comparable frequencies (at limiting dilution) upon serial transplant [71].
CD133/AC141⁺CD44⁺	DU145 cells (monolayer and sphere culture)	Purified cells display increased tumor-initiating ability in male IC mice [69].
CD44⁺ integrin α₂β₁⁺	Human PCa cell lines (monolayer cell culture)	CD44⁺ PCa cells are more tumorigenic than CD44− cells; CD44⁺ integrin α₂β₁⁺ cell population is enriched in PCSCs [60, 61].
CD44⁺CD24⁺ integrin α₂β₁⁺	DU145 spheres	Sphere cells display increased tumor-initiating ability in male IC mice [70].
CD44⁺ALDH^hi integrin α₂β₁⁺	LAPC9 xenograft (SC; castrated male, IC mice)	10¹ sorted PCa cells form 2° tumors in castrated IC mice compared to 10⁴ CD44-ALDH^low integrin α₂β₁⁺ - cells [91].
TRA-1-60⁺CD166⁺CD151⁺	CWR22 xenograft (OT; intact male, IC mice	Triple marker-positive PCa cells display enhanced tumor-initiating ability compared to TRA-1-60⁺ cells [72].
HLAI⁻	Human primary PCa cells and cell lines	HLAI⁻ PCa cells display increased tumorigenic potential in IC mice compared to HLAI⁺ cells [162].

Abbreviations: 1° primary; 2° secondary; hi high activity; HLAI human leukocyte antigen class I; IC immunocompromised; OT orthotopic; PCa prostate cancer; TS testosterone-supplemented; SC subcutaneous.