Figure 6. Glycerol derivatives and phospholipids are dependent on HIF1α.

(a) TAG normalized levels detected by ¹H-NMR in the organic phase of the cell extracts, reported as mean ±sd (n=3). (b) Glycerol normalized levels detected by ¹H-NMR in the aqueous phase of the cell extracts, reported as mean ±sd (n=3). (c) Glycerophosphate normalized levels detected by CE/MS in the aqueous phase of the cell extracts, reported as mean ±sd (n=3). (d) Metabolic pathway generating the precursors utilized in the Kennedy pathway. Abbreviations:CMP, Cytidine monophosphate; Pi, phosphate inorganic; CDP-choline, Cytidine-diphosphocholine; PLD3, Phospholipase D3. (e) Choline, (f) phosphocholine, (g) phosphatidylcholine and (h) MAG normalized levels reported as mean ±sd, were detected by ¹H-NMR in the organic and aqueous phase of the cell extracts (n=3). (i) Phospholipase D3 normalized levels detected by label-free quantitative proteomics analysis in HCT116 cells data are reported as mean ±sd (n=3).