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. 2014 Dec 3;6(4):2101–2119. doi: 10.18632/oncotarget.2937

Figure 4. EPOR inhibition increases the polyploidy of U87 cells after radiation or temozolomide treatments.

Figure 4

U87 cells infected with scrambled shRNA or EPOR shRNA were treated with a single dose of X-rays (8 Gy) or TMZ (100 μM) 24 hours after seeding cells. At different times after treatment exposure (0h, 14h, 24h, 48h, 72h and 120h for X-rays and 0h, 24h, 48h, 72h and 120h for TMZ), cells were incubated with propidium iodide to realise a fluorescence-activated cell sorting (FACS)–based cell cycle analysis. Distribution kinetics of different cell cycle phases after X-rays treatment (8 Gy) and TMZ treatment (100 μM) of U87-scrambled (A and C, respectively) and U87-shEPOR (B and D, respectively). N=1 for each time point for both X-rays and TMZ treatments.