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. 2014 Dec 10;6(4):2148–2163. doi: 10.18632/oncotarget.3236

Figure 5. The effects of tylophorine compounds in c-Myc regulated Warburg effect and P-body (processing body) formation.

Figure 5

A. Tylophorine treatment decreased the protein expressions of GLS1 and LDHA involved in Warburg effect as well as eIF4E and DCP1a in P-body formation. B Ectopically Overexpressed c-Myc restored the protein expressions of GLS1 and LDHA. The relative expression levels of each protein were normalized with their respective internal control GAPDH. C. Tylophorine treatment decreased the formation of P-bodies. The P-bodies were visualized by immunofluorescent staining using anti-Dcp1a-TRITC (in red) and eIF4E-FITC (in green). DAPI (in blue) was used for nuclear counterstaining. The carcinoma cells were treated with DMSO, tylophorine (2 μM), or nocodazole (30 ng/mL) for 24 h prior to western blotting or immunofluorescent staining analyses with the indicated antibodies. The percentage of the cell population exhibiting P-bodies and the relative percentages of the total number of P-bodies in each treatment were counted with Image-J software (National Institutes of Health) and listed. Nocodazole was used an additional compound control that induces formation of P-bodies. The results shown are representative of 3 independent experiments.