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. 2014 Dec 11;6(4):2164–2179. doi: 10.18632/oncotarget.2941

Figure 2. Downregulation of CIP2A determined the effects of afatinib on p-AKT and apoptosis in NSCLC cells through PP2A activation.

Figure 2

(A) Dose-dependent effects on CIP2A, p-AKT and AKT. (B) Effects of afatinib on PP2A activity. NSCLC cells were exposed to afatinib at the indicated concentrations for 24 h. Data are mean ± SD. n = 3 for each experiment. **, p < 0.01, vs. no afatinib. (C) Time-dependent effects of afatinib on CIP2A, p-AKT and apoptosis-related proteins in the sensitive H358 and resistant H460 cells. Cells were exposed to 10 μM afatinib for up to 48 hours. Immunoblots were scanned and quantitated to determine the ratio of the level of CIP2A to actin. Data are mean ± SD. n = 3 for the different time intervals.