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. 2014 Dec 10;6(4):2235–2249. doi: 10.18632/oncotarget.2946

Figure 6. βIII-tubulin silencing reduces both anchorage dependent and independent pancreatic cancer cell growth.

Figure 6

A) xCelligence assay of MiaPaCa-2 cells transfected with ns-siRNA or βIII-tubulin siRNA (βIII-Tub siRNA). 72h post-transfection cells were seeded into xCelligence plates. Circles represent cell index (mean±s.e.m.) at hourly time points, and are directly related to cell number. Asterisks indicate significance (*** p≤0.001; n=3). B) MiaPaCa-2 cells transfected with mock, ns-siRNA or βIII-Tubulin siRNA were embedded in soft-agarose at 48h post-transfection and allowed to form colonies. Bars represent the number of colonies that formed (mean+s.e.m. as a % of mock). Asterisks indicate significance (*p≤0.05; n=3). C) As per B, except experiments were carried out with HPAF-II cells (* p≤0.05; n=3). D) MiaPaCa-2 cells were transfected with ns-siRNA or βIII-tubulin siRNA and 24h post-transfection cells were cultured under anchorage independent conditions for a further 48h (wells coated with Poly-HEMA). Bars represent the apoptotic fraction determined by Annexin V and 7AAD staining (mean±s.e.m.). Asterisks indicate significance (*** p≤0.001; n=3).