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. 2014 Dec 11;6(4):2509–2523. doi: 10.18632/oncotarget.3191

Figure 2. In vivo neddylation inhibition blocks tumor progression.

Figure 2

(A) Representative Western blot analysis of global neddylation (shown as neddylated cullins) in whole extracts from WT and Phb1-KO livers and isolated hepatocytes. (B) Reduction of tumor size in Phb1-KO mice after MLN4924 treatment determined by caliper measurement of the longest diameter in the transverse view of the liver. (C) Staining of liver sections from Phb1-KO MLN4924 treated and Phb1-KO untreated mice with Nedd8 and HuR antibodies. Graphical representations are shown on the right of each panel. (D) Western blot analysis of Nedd8 and HuR on liver lysates from Phb1-KO MLN4924 and Phb1-KO untreated animals. (Values are mean ± SEM. *p<0.05; Phb1-KO MLN4924 vs Phb1-KO).