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. 2014 Dec 11;6(4):2509–2523. doi: 10.18632/oncotarget.3191

Figure 7. LKB1 and Akt stabilization play an important role on neddylation-induced metabolic disruptions in liver cancer.

Figure 7

(A) Western blot analysis against Nedd8, LKB1 and Akt in Phb1-KO hepatocytes overexpressing Akt and LKB1 after 48 hours of MLN4924 treatment. (B) Basal oxygen consumption rate (OCR) and (C) extracellular acidification rate (ECAR) values in Phb1-KO hepatocytes after LKB1 and Akt overexpression and silencing. (D) OCR and ECAR were measured in Phb1-KO hepatocytes overexpressing LKB1 and Akt after 48 hours of MLN4924 treatment. The energetic response in cells was measured in the presence of oligomycin, FCCP and rotenone. (E) Caspase-3 activity in Phb1-KO hepatocytes overexpressing LKB1 and Akt after 48 hours of MLN4924 treatment. Values are mean ± SEM. *p<0.5, ***p<0.001 (LKB1/Akt overexpression and silencing vs control).