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. 2014 Oct 24;6(4):2524–2538. doi: 10.18632/oncotarget.2629

Figure 5. β-catenin is a molecular target of Vav/JNK activation.

Figure 5

a. β-catenin is a target of JNK. Total cell extracts from stable NIH3T3 cell lines cultured in the presence (+) or not (−) of SP600125 were analysed by sequential immunoblotting with the indicated Abs. Fold increase of phospho-β-catenin in cells expressing wt-Vav1, onco-Vav and D797N compared to control vector were normalized to whole β-catenin. b-c. Vav1 and β-catenin are interacting partners. b. Lysates from the indicated stable NIH3T3 cell lines were immunoprecipitated with anti-Myc Tag Ab. Immune complexes (upper panels) or total extracts (lower panels) were analysed by sequential immunoblotting with the indicated Abs. c. GST-fusion proteins as described in the upper panel were incubated with NIH3T3 cell extracts. Interacting β-catenin was determined by immunoblotting. Total cell extract (Input) was used as a control for β-catenin expression and GST fusion proteins were detected using Ponceau red. GST-SH* corresponds to D797N mutation introduced into GST-SH fusion protein.