FIG. 2.

Heat shock induction of dnaK. Transcriptional map and Northern hybridization of RNAs from samples taken at the indicated times. The heat shock pattern was as indicated for Fig. 1A. The probes are indicated by solid lines. The sizes of the hybridizing bands (in kilobases) are indicated by arrows on the left and right of the panels. Putative transcriptional terminators are shown by stem-loop structures. 16S rRNA was hybridized as a control. Observe the heterogeneity present in the smear because of the interference with the rRNAs. The “omega” symbols (palindromes) represent putative terminators found in the 3′ ends of the grpE and hspR genes. The medium used for RNA extraction was TYG (2× TY plus 2% glucose [see the text]).