Fig. 1.

Order of events for assessing human pancreatic islets. A: islets isolated from donor pancreata at isolation centers were shipped by overnight courier to Vanderbilt, where they were hand-picked for further purity and IEQ quantification. Islets were perifused to assess in vitro function. Islets were used for subsequent studies that are not part of the current report. B: images showing a human islet preparation before (top left) and after (top right) hand picking. Immunolabeling of human islets for DAPI (blue), insulin (green), and glucagon (red) embedded in collagen gel (far right).