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Northern blots carried out with 5 μg of RNA isolated from either the L. casei wild-type strain BL23 (WT) or one of the five CCR-relieved mutants grown in MRS fermentation medium complemented with glucose. A levA-specific probe was used to detect the lev operon transcript of 3.4 kb in the various samples. The positions of 16S and 23S rRNAs are indicated with arrows.
