FIG. 9.
Phosphoryl group transfer from [32P]P∼His-HPr (A) and [32P]P∼His-HPrI47T (B) to EIIALev. [32P]PEP, EI, and HPr or HPrI47T were preincubated to allow their exhaustive phosphorylation before EIIALev was added to the assay mixtures, which were further incubated at 37°C. Aliquots were withdrawn after different time periods and separated on 0.1% SDS-15% polyacrylamide gels which were dried and exposed to autoradiography. The migration positions of EI, HPr, HPrI47T, EIIALev, and its dimer are indicated by arrows.