TABLE 1.
Strains used in this study
| Strain | Genotype or characteristica | Source or reference |
|---|---|---|
| E. coli | ||
| Fit | F−ompT hsdSB(rB− mB−) gal dcm (DE3) ptsH | Takara Bio Inc. |
| DH5α | F− φ80lacZ ΔM15 Δ(lacZYA-argF)U169 deoR endA1 recA1 hsdR17(rK− mK−) supE44 thi-1 gyrA96 relA1 phoA | Invitrogen |
| NM522 | SupE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM) (rK− mK−) [F′ proAB lacIqZΔGM15] | 25 |
| L. casei | ||
| BL23 | Devoid of pLZ15 | 1 |
| BL71 | ccpA::erm | 39 |
| BL121 | ptsH1 | 51 |
| BL122 | ptsH2 | 51 |
| BL123 | ptsH3 | 51 |
| BL124 | ptsI::erm | 51 |
| LcG102 | hprK::pHKLc208(hprK208Am) | 15 |
| BL23levB | levB10(Oc) | This work |
| BL23H3levB | ptsH3 levB10(Oc) | This work |
| BL23levX | levX5(Oc) | This work |
| BL23H3levX | ptsH3 levX5(Oc) | This work |
| BL23levR | levR::pRVlevR | This work |
| BL23H3levR | ptsH3 levR::pRVlevR | This work |
| BL23sig54 | rpoN19(Oc) | This work |
| BL23H3sig54 | ptsH3 rpoN19(Oc) | This work |
a
Am and Oc indicate amber (UAG) and ochre (UAA) stop codons.