TABLE 2.
Plasmids used in this study
| Plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| pBluescriptSK(+) | Amp | Invitrogen |
| pCDNA2.1 | Amp | Invitrogen |
| pQE30 | Amp | QIAGEN |
| pQELevA | levA cloned in pQE30 | This work |
| pQELevB | levB cloned in pQE30 | This work |
| pET-15b | Ampr | Novagen |
| pET-ptsH | ptsH cloned in pET-15b | C. D. Esteban, unpublished |
| pET-ptsH3 | ptsHI47T cloned in pET-15b | This work |
| pMal-c2X | Amp | New England BioLabs |
| pMalLevR | levR cloned in pMal-c2X | This work |
| pMalLevR-H1 | levRH1 cloned in pMal-c2X | This work |
| pMalLevR-H12 | levRH12 cloned in pMal-c2X | This work |
| pRV300 | Erm and Amp | 31 |
| pRVlevR | Internal fragment of levR cloned in pRV300 | This work |
| pRVlevB | levB allele with stop codon 10 cloned in pRV300 | This work |
| pRVlevC | levC allele with stop codon 6 cloned in pRV300 | This work |
| pRVsig54 | rpoN allele with stop codon 19 cloned in pRV300 | This work |