TABLE 3.

Oligonucleotides used in this study

Function and name	Sequencea	Used for:
Construction of mutants
LvRXh	ACGCGCTCGAGATCAAAGATGTGATTCTTGC	Cloning part of levR
LvRPs	CCAACTGCAGGAATCACAACTAATCAGGCT
LvBBlunt	ACCGCAATACTGTCCGAAC	Cloning levB and inserting stop codons and restriction sites
LvBMut1	CGTATTGATTAACGCATGCTTTAAGGCTTGATCACGGTTCGT
LvBMut2	ATCAAGCCTTAAAGCATGCGTTAATCAATACGTACAAACGAA
LvBNh	AACCGCTTGGCTAGCCATTCATGAGTTGGAACATAAG
LvXKp	GACGGCGGTACCATTAACGATATGCGT	Cloning levX and inserting stop codons and restriction sites
LvXMut1	TAGACTAAGCTTTAAGAAAAAGAAGTTGCGTATCAG
LvXMut2	TAAAGCTTAGTCTATTAGTCAATTGTCATTTTGATTTT
LvXEc	GCCGGAATTCCTTCACCGGATTGTC
SLSp	GTTGGTACTAGTGATCCGCACATTA	Cloning part of rpoN and inserting stop codons and restriction sites
SLMut1	ACGTAAGGGATCCGATAGTAAATTCAGATGTTGCAAT
SLMut2	TTTACTATCGGATCCCTTACGTTAGCACCAACTTATTG
SLBlunt	TCTGCAAATACCAGCTCAG
Overexpression of genes
QEABA	AGGGATGGGATCCATGAAATATTTGCTTCTTG	Cloning levA
QEAKP	TTTTGCTGGTACCTTAAATATCGTCGTCGTC
QEBBA	AGGAGTGGGATCCATGGCAATTTCGTTTGTA	Cloning levB
QEBKP	TATTGTGGTACCATTAATACCCAAAGCG
H3-1	CAGATCACATATGGAAAAACGCGAAT	Cloning ptsHI47T
H3-2	GCAGAGATCTCCTTCAAATGTTCAG
MalRXBA	GGTGTCTAGAATGAACGCAGTAGAAAAACTTTATG	Cloning levR
MalRPST	CCTTTTTGTCTGCAGATCACGCCACAAAAATAGAGT
MH1A	TGTGTTCGCCGGCAGCAAGCAGGATGGCATTGTA	levR His488Ala mutation
MH1B	ATCCTGCTTGCTGCCGGCGAACACACCGCATCGAG
MH2A	TTAAGGACAAGGCCATGAAGAGATTGAGCTTTAG	levR His776Ala mutation
MH2B	AATCTCTTCATGGCCTTGTCCTTAATGATTGAACG
Detection of the transcriptionnal start site
EIIA2	GTTTGCTTTAAGCCACTGG	Identification of the transcription start site of the lev operon
EIIA5	CATCAATGTGGTTAACGCCA
EIIA6	CCAGAACAACAAAGCTTGCA
levR5	ACGACGAATGGCTTCGTG	Identification of the transcription start site of levR
levR6	TGCTTCGATTGCCAGCTG
levr7	CATCGGCAGCCTGATTAG

^aLetters in boldface type indicate newly created restriction sites; newly created stop codons or mutations are underlined.