Fig. 6.
Effects of IR and IGF-1R inhibitors PPP and AG-1024 on ENaC-mediated currents stimulated by insulin and IGF-1. A: summarized time course of the action of the IGF-1 inhibitor PPP (500 nM, overnight treatment) on 100 ng/ml IGF-1-stimulated ENaC-mediated current. Amiloride (10 μM) was added at the end of experiments to ensure that the currents recorded were ENaC driven. Each summarized point on the representative graph stands for at least n = 6. B: AG-1024 inhibited both IGF-1- and insulin-simulated currents in the mpkCCDcl4 cell line. Shown is a summarized time course of the action of the IGF-1 inhibitor AG-1024 (500 nM, applied before the experiment) on IGF-1-stimulated (12 nM) and insulin-stimulated (20 nM) ENaC-mediated currents. Each summarized point on the representative graph stands for at least n = 6.