Fig. 8.

Effect of TEA, 4-AP, and Ba²⁺ on the outward currents in isolated HGCS cells. A and B: a representative trace showed outward currents during ramp depolarization (from −80 to +80 mV; see inset) from a holding potential of −80 mV. TEA (10 mM) did not show significant effect on evoked outward currents (A). However, 4-AP (5 mM) decreased the outward currents (B). C: a representative trace showed the effect of Ba²⁺ (100 μM) after replacement with K⁺-rich solution (HK; see methods) at −80 mV. Vertical lines denote ramp depolarization and blue dotted line denotes the holding current at −80 mV. D: a (black line) and b (red line) are evoked currents during ramp depolarization from C in control and HK presence, respectively. E: bath application of Ba²⁺ (100 μM, c: green line) under external K⁺-rich solution (b: red line) at a holding potential of −80 mV did not reveal significant effect on ramp-evoked currents.