Table 1. Cell death was monitored in U251 after irradiation (5 Gy), ETO (50 μg/ml), TRAIL (50 ng/ml), FasL (60 ng/ml) or cisplatin (15 μg/ml) treatment after 5, 24, 48 and 72 h by FACS analysis using propidium iodide incorporation and caspase-3 activation.
| CTR | 6 h | 24 h | 48 h | 72 h | |
|---|---|---|---|---|---|
| Etoposide | |||||
| % Dead cells | 2.6±0.8 (n=3) | NA | 28±2 (n=4) | 28±3 (n=4) | NA |
| DEVDase activity | 3.3±0.3 (n=3) | 37±11 (n=4) | 204±18 (n=4) | 128±11 (n=4) | NA |
| Irradiation | |||||
| % Dead cells | 2.6±0.8 (n=3) | NA | 6±2 (n=3) | 8±1 (n=3) | 15±1 (n=5) |
| DEVDase activity | 3.3±0.3 (n=3) | NA | 65±25 (n=3) | NA | 143±13 (n=5) |
| TRAIL | |||||
| % Dead cells | 2.6±0.8 (n=3) | 22±3 (n=4) | 6.4±1 (n=3) | NA | NA |
| DEVDase activity | 3.3±0.3 (n=3) | 107±3 (n=4) | 7±0.3 (n=3) | NA | NA |
| FASL | |||||
| % Dead cells | 2.6±0.8 (n=3) | 10±1 (n=3) | 20±3 (n=3) | NA | NA |
| DEVDase activity | 3.3±0.3 (n=3) | 38±8 (n=3) | 115±3 (n=3) | 51±13 (n=3) | NA |
| Cisplatin | |||||
| % Dead cells | 2.6±0.8 (n=3) | NA | 16±2 (n=3) | NA | NA |
| DEVDase activity | 3.3±0.3 (n=3) | NA | 139±18 (n=3) | NA | NA |
Abbreviation: NA, not applicable
Apoptosis was measured through caspase-3 activation