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. 2004 Jul;186(14):4774–4780. doi: 10.1128/JB.186.14.4774-4780.2004

FIG. 1.

FIG. 1.

Comparison of flavonoid-induced extracellular proteins secreted by Rhizobium sp. strain NGR234, R. fredii USDA257, and various mutant strains. (A) Silver-stained SDS—15% PAGE gel of secreted proteins of NGR234 (lanes 1 and 2) and USDA257 (lanes 3 and 4) collected after 40 h of induction with apigenin (lanes 1 and 3) or genistein (lanes 2 and 4). (B) Western blot of flavonoid-induced secreted proteins of NGR234 (lanes 1 and 2), USDA257 (lanes 3 and 4), NGRΔnopX (lane 5), NGRΩnopL (lane 6), and the double mutant NGRΔnopXΩnopL (lane 7) separated on an SDS—12% PAGE gel and immunostained with SR3 antibodies. Cells were induced with either apigenin (lanes 1, 3, 5, 6, and 7) or genistein (lanes 2 and 4). The positions of NopX, NopL, and SR3 are indicated by arrows. Estimated molecular masses (in kilodaltons) are indicated on the left.