FIG. 2.

NopP cross-reacts with SR3 antibodies, and its secretion requires a functional TTSS. (A) Silver staining of an SDS—12% PAGE gel containing extracellular proteins of apigenin-induced strains NGR234 (lane 1), NGRΔnopP (lane 2), and NGRΩrhcN (lane 3). (B, C, and D) Western blots of identical gels immunostained with SR3, NopP, and SRT antibodies, respectively. The positions of NopP (P), NopL (L), and NopX (X) are indicated by arrows. Estimated molecular masses (in kilodaltons) are indicated.