TABLE 1.
Homologies of overlapping clones determined by phage display with SR3 antibodiesa
| Overlapping clonesb | Best BlastX match
|
Best BlastN match
|
Orientationc | ||
|---|---|---|---|---|---|
| Sequence | % Similarity | Sequence | % Identity | ||
| SR3c13 (81) and SR3ce6 (73) | y4yP (NGR234) | 100 | pNGR234a | 100 | Correct |
| SR3c14 (202) and SR3c11 (52) | FlaB (A. tumefaciens) | 57 | None | Correct | |
| SR3c21 (132) and SR3c22 (118) | Smb21498 (Rhizobium meliloti) | 91 | Rhizobium meliloti pSymB | 90 | Inverted |
| SR3c1 (88) and SR3cb5 (56) | Smc04127 (Rhizobium meliloti) | 100 | Rhizobium meliloti chromosome | 96 | Inverted |
a
Blast searches were performed by using GenBank nonredundant databases.
b
The numbers in parentheses are sizes of the inserts (in base pairs).
c
Compares the orientation of the peptide encoded by the phage insert with that of the best match identified in BlastX searches.