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. 2015 Mar 12;6(3):e1688. doi: 10.1038/cddis.2015.56

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression and activity of the Cripto-Nodal signaling pathway in postnatal mouse spermatogonia. (a) Semiquantitative RT-PCR analysis of mRNA expression of the indicated elements of the Cripto-Nodal signaling pathway in Kit+ and Kit- spermatogonia. (b) Western blot analysis of Smad2 phosphorylation in cultured untreated Kit+ spermatogonia and in the same cells treated overnight with recombinant Nodal, in the presence or absence of the Alk4/7 selective inhibitor SB431542. Densitometric analysis of western blots from three independent experiments is shown below. Bars represent the mean ±S.D.