Figure 5.

TSP50 promoted PMA-induced MMP9 expression. (a) The MMP2 and MMP9 activities in the media from either control or TSP50-expressing cells treated by PMA were examined by gelatin zymography as described in materials and methods. The activity of a recombinant MMP9 and MMP2 protein containing pro- and active band was used as a positive control. (b) Effects of TSP50 overexpression on MMP2 expression were analyzed by western bloting. (c) Effects of TSP50 overexpression on MMP9 expression were analyzed by western blotting. (d) Induction of MMP9 mRNA expression by TSP50. The MMP9 mRNA levels were measured by RT-PCR using β-actin as an internal control. (e) Increased in situ gelatinolytic activity by TSP50. The control and TSP50-expressing cells were processed for in situ zymography as described in materials and methods, and 20 mM of GM6001 was used to inhibit MMPS in TSP50-expressing CHO cells. (f) Effects of TSP50 expression on p-c-jun nuclear translocation. Scale bar, 150 μm. (g) Effects of TSP50 expression on MAP kinase activities