Skip to main content
. 2015 Mar 26;6(3):e1702. doi: 10.1038/cddis.2015.69

Figure 4.

Figure 4

Loss of TDP-43 strikingly promotes the production of PGE2 in microglia but not in astrocytes. (a) Primary cultured microglia were transfected with si-control and si-TDP-43 for 96 h. Then, the media from for 24 h cultures of microglia were subjected to PGE2 enzyme-linked immunosorbent assays (ELISAs). The data from three independent experiments are presented as the means±S.E.M.; **P<0.01; one-way ANOVA (b) BV2 cells and primary cultured astrocytes were transfected with si-control or si-TDP-43 for 72 h or 96 h at a final density of 1 × 106 cells per well in 12-well plates. Then, the 1 : 10 diluted media from 24 h cultures of BV2 cells or primary cultured astrocytes were subjected to PGE2 ELISAs. The data from three independent experiments are presented as the means±S.E.M.; ns, not significantly different; **P<0.01; one-way ANOVA. (c) BV2 cells were transfected with si-control or si-TDP-43 for 48 h. Then, the cells were treated with DMSO or U0126 (20 μM) for 24 h, with a final cell density of 3 × 105 cells per well in 24-well plates. Next, the media from 24 h cultures of BV2 cells were subjected to PGE2 ELISAs. The data from three independent experiments are presented as the means±S.E.M.; ns, not significantly different; **P<0.01; one-way ANOVA. (d) Similar experiments as in (b) were performed, but the BV2 cells were treated with celecoxib (50 μM) instead of U0126. The data from three independent experiments are indicated as the means±S.E.M.; ns, not significantly different; **P<0.01; one-way ANOVA