TABLE 2.
Plating of P2 and related phages on strains carrying the rpoC397 and rho(Ts)15 mutations
| Phage | Efficiency of platinga
|
|||
|---|---|---|---|---|
| P90A5c (rpoC+) | 397c (rpoC397) | SA1030 (rho+) | AD1600 [rho(Ts)15] | |
| P2 vir-1 | 1.0 | 1 × 10−7 | 1.0 | 4 × 10−9 |
| 186 cI(Ts) | 1.0 | 1.0 | 1.0 | 1.0 |
| Hy5 (P2/186 hybrid) | 1.0 | 1 × 10−7 | 1.0 | <1 × 10−9 |
| P2 vir-1 trl-1 | 1.0 | 1.0 | 1.0 | <1 × 10−7 |
| P2 vir-22 L(Ts)37 gor-3b | 1.0 | 1.0 | 1.0 | 0.21 |
All platings were performed at 33°C.
The vir-22 and L(Ts)37 mutations do not affect P2 growth under the conditions used and were employed to facilitate mapping of the gor mutation. Hy5 also carries the 186 cI(Ts) mutation.