Figure 3.

Forced expression of HEY1 promotes invasion of Mesenchymal stem cell. HEY1 expression vector-transfected mesenchymal stem cell or control vector-transfected mesenchymal stem cell were examined by using an invasion assay. (A) Expression of HEY1 was determined using western blotting. (B) Column graphs show percent of invading cells after the invasion assay. Percent of invading cells in control vector-transfected cell were defined relative to an invasion of 100%. Representative photomicrographs show invaded cells. Scale bar is 200 μm. *P<0.05, Student's t-test. (C) Real-time PCR and invasion assays were performed to examine the relationship between HEY1 expression and invasion. P<0.01, Pearson correlation coefficient.