Table 3.
Summary of weighted mean open and closed dwell-times
|
τO |
τC |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| LTH |
NX |
LTH |
NX |
||||||
| Phosphorylation State | Adult | Fetus | Adult | Fetus | Adult | Fetus | Adult | Fetus | |
| Native | 3.84 (8) | 13.81 (7) | 1.84 (5) | 2.09 (6) | 8.32 | 27.03 | 6.64 | 6.69 | |
| Dephosphorylated | 2.77 (6) | 9.65 (6) | 1.66 (5) | 1.63 (6) | 8.59 | 21.07 | 26.37 | 19.88 | |
| PKA | 1.31 (5) | 1.83 (7) | 1.73 (6) | 1.48 (7) | 14.61 | 4.98 | 8.71 | 5.48 | |
| PKG | 1.33 (6) | 0.92 (5) | 1.45 (5) | 1.85 (5) | 6.19 | 13.89 | 2.06 | 28.83 | |
Dwell-times were obtained from square-root versus logarithmic time plots (Fig. 4, B and C) best fitted to an exponential function with 3 components. Mean time constants (τ) were multiplied by corresponding weighing factor (see Supplemental Table S1, including P values), and the 3 products were summed to yield weighted mean open (τo) and closed (τc) times for different phosphorylation states: native control; dephosphorylated (alkaline phosphatase, Apase); and phosphorylation by added PKA and PKG following pretreatment with Apase. Sample sizes (n) are shown in parentheses.