Table 1.

Working dilutions and immunoblot buffers of the employed antibodies

Antibody	Supplier	Dilution	Blocking Buffer	Antibody Incubation Buffer
Primary antibodies
phospho-AMPKα (Thr-172) (40H9); rabbit	Cell Signaling	1:1000	5 % BSA/TBST 50 mM NaF	5 % BSA/TBST 50 mM NaF
AMPKα (23A3); rabbit	Cell Signaling	1:1000	5 % BSA/TBST	5 % BSA/TBST
phospho-CREB (Ser-133); rabbit	Biotrend/Signalway	1:4000	3 % BSA/TBST 50 mM NaF	3 % BSA/TBST 50 mM NaF
CREB (Ab-129); rabbit	Biotrend/Signalway	1:4000	3 % milk/TBST 50 mM NaF	3 % milk/TBST 50 mM NaF
CBS monoclonal (M01), clone 3E1; mouse	Abnova	1:1000	5 % milk/PBST	5 % milk/PBST
CSE monoclonal (M03), clone  S51; mouse	Abnova	1:1000	5 % milk/PBST	5 % milk/PBST
α-tubulin; mouse	Sigma	1:3000–1:10000	3–5 % milk/TBST	3–5% milk/TBST
Secondary HRP-linked antibodies
anti-rabbit IgG	Cell Signaling	1:3000	n/a	primary antibody buffer
anti-mouse IgG	Thermo Scientific	1:3000	n/a	primary antibody buffer

TBST, Tris-buffered saline containing 1 % Tween-20; PBST, phosphate-buffered saline containing 1% Tween-20; BSA, bovine serum albumin; CSE, cystathionine-γ-lyase.