Table 1.
Sequences of primers for quantitative PCR
| Sequences of Primers for Quantitative PCR |
|||
|---|---|---|---|
| Target | PubMed Accession No. | Forward primer | Reverse primer |
| A1AR | NM_204316.3 | GAAGATCCCGGTCAGGTATAAGAG (327–350) | GGTTATTCCAACCGAACATTGG (424–445) |
| A2AAR | XM_425280.4 | GCAGCTCAAGCAGATGGAGAAC (645–666) | TGGCTGCGTGGACTTCCTT (706–724) |
| A2BAR | NM_205087.1 | GCTGCTTTTCGTGTCTCTTTGAG (497–519) | CAGCCGAAGAAGTTGAAGTATACCA (542–566) |
| A3AR | NM_204151.1 | CCACATGACATTAAGCCCAAAA (1107–1128) | AGCGACAACCAAGCCAAATT (1167–1186) |
| CD39 | NM_204247.1 | GCT GGT CGA TGG CTA TAA GTT TG (1230–1252) | CTG CGT TTC CTG CCT TCT G (1285–1303) |
| CD73 | XM_419855 | TGC TTT GTG ATG CTA TGC TTT ATG (1052–1075) | AGT GAA ACA TGG TTC CAC GAC TT (1099–1121) |
| ADA | NM_001006290.1 | AAA GGG ATT TCC GCA TCA AA (425–444) | CTG GAG ACC AAC TTG GCA TAT (474–493) |
| AdK | NM_001006501.1 | GAGACGGAAGCTGCCACTTTT (658–678) | CGGGCTATCTCTTTAATGTCTTCAG (701–725) |
| ENT1 | XM_419491.3 | GGCCATCTTCTCCTACGTTCTTC (627–649) | GGTACTCTGTCTTGTCCTTCATGGA (679–703) |
| ENT3 | XM_421594.3 | AACCGGCCCCCTCTGA (218–233) | CGGTCGAAGCGATGGAGAT (259–277) |
| ENT4 | XM_003642144.1 | CGC ATC TCT GTG GGC TAC CT (352–371) | GTC GCA GAT GCT GAC GAA GC (392–411) |
| CNT3 | XM_425033.3 | GTG GGA GCA TGT ACG TAA ATG G (1295–1316) | AAA GAG CAT AGG TGG CAA TGG T (1348–1369) |
| GAPDH | NM_204305.1 | GTG GAG GGT CTT ATG ACC ACT GT (489–511) | GGG CCA TCC ACC GTC TTC (533–550) |
| HIF1α | NM_204297.1 | CACACCATGATATGTTCACGAAA (866–888) | AACCCAGACGTAGCCACCTT (929–948) |
See text for additional details.