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. 2015 Mar 26;10(12):1974–1981. doi: 10.1016/j.celrep.2015.02.060

Figure 3.

Figure 3

NMN and NAD Changes Resulting from a Lack of NMNAT2 during Development Occur in the Absence of SARM1

(A) NMN and NAD levels (nmol/g tissue) and NMN/NAD ratios in brains from E18.5 embryos/P0 pups of the indicated genotypes. Means ± SEM are plotted (n = 5–7 each genotype). Data for samples wild-type for Sarm1 (red tones) have been presented previously in a different format (Di Stefano et al., 2014). Re-use of the same dataset here allows direct comparison with the Sarm1−/− background data (blue tones), which was obtained in parallel.

(B) NMN and NAD levels (nmol/mg extracted protein) and NMN/NAD ratios in separate neurite or ganglia fractions from 7-days-in-vitro explant cultures of DRGs from E13.5/14.5 embryos of the indicated genotypes. Ganglia fractions contain short proximal neurite stumps as well as cell bodies. Means ± SEM are plotted (n = 3–5 each genotype). See also Figure S1, where data in (B) are presented as relative changes.