Figure 1.

New Talin Mutants

(A) The diagram shows the domains of talin: F0–F3 of the FERM domain (yellow), vinculin-binding sites (light gray), integrin-binding site 2 (dark blue), and actin-binding domain (red). IBS2 is also a VBS. Mutations are indicated above talin: 38 cause a truncation (black), and one causes a substitution (R367H; blue). The truncations are named according to their last in-frame talin residue (see Table S1).

(B) GFP-talin and Δhead transgenes. The position of GFP (green) and deletion (dashed line) are indicated.