Figure 4.

Both Talin Head and IBS2 Are Used for Wing Adhesion

(A) Representative pictures of newly hatched flies with normal wings (top) or one wing with a blister (bottom; pink arrow).

(B) Percentage of blistered wings in flies with homozygous mutant clones for the mutants indicated (for all mutants, see Figure S3), from ≥100 flies/mutant. Bar colors show four statistically distinct categories (^∗p < 0.01). Flat bars (horizontal lines) indicate no defect. Genotypes not analyzed do not have a bar.

(C) Phenotypic differences between muscle and wing are not explained by differences in protein levels, determined by western blotting of talin⁶⁴⁶ and talin²⁵⁰⁹ (top histogram) and talin site-directed mutants (bottom histogram) in embryos (light gray) or pupal wings (dark gray) heterozygous for the talin mutation. The protein levels were normalized to wild-type talin in each sample. SD is shown from two independent experiments.