Figure 2.

Brain Injury Modulate Fitness Fingerprints of Adult Neurons

(A) Different isoforms of the cell membrane protein Flower (ubi, LoseA, and LoseB) form tissue-specific fitness fingerprints. Identical regions are depicted in gray.

(B) Expression of Flower^LoseA and Flower^LoseB in intact OLs in green and red, respectively. Staining for Flower^ubi is shown in white. DAPI marks nuclei (blue).

(C and D) Insets show Flower^ubi (C) and Flower^LoseA expression (C and D) on mature neurons (Elav⁺; white) (D).

(E and F) Flower^LoseB (red) induction in laterally punctured OLs 24 hr and 48 hr AD.

(G) Flower^LoseB expression (red) in laterally injured OLs 72 hr AD.

(H) At 48 hr after injury, Flower^LoseB (red) is upregulated in injury-exposed neurons (Elav⁺; white). Arrowheads point to neurons with induced Flower^LoseB expression, and the arrow shows nuclear accumulation of Flower^LoseB in a dying neuron (small nucleus; Elav⁺). Flower^LoseA (green) shows panneuronal expression.

(I) Scheme illustrating the change in Flower fitness fingerprints upon damage.

Dashed arrows mark needle insertion sites. Scale bars represent 10 μm (C, D, and H) and 20 μm (B and E–G). See also Figures S2 and S3.