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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2015 Jan 22;0:49–56. doi: 10.1016/j.prostaglandins.2015.01.001

Figure 1. Atf3 negatively regulates Ptgs2 expression in macrophages.

Figure 1

(A, B) Levels of Ptgs2 and Atf3 mRNA in resident peritoneal macrophages stimulated without or with zymosan for 3 or 6h. (C) Expression of Ptgs2 mRNA in unstimulated resident peritoneal macrophages isolated from WT or Atf3−/− mice. (D) Levels of Ptgs2 mRNA in resident peritoneal macrophages isolated from WT or Atf3-deficient mice and stimulated with zymosan for 3 or 6h. (E) Western blot of COX-2 protein in WT or Atf3-deficient macrophages stimulated with zymosan for 6h, with quantification shown in the lower panel. (F) PGE2/D2 levels in supernatants of WT and Atf3−/− macrophages stimulated without (control) or with zymosan (6h). Results are mean ± SEM, n=3–7/group. *P<0.05; NS=not significant