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Characterization of ESCs. ESCs were purified from endometrial samples and their characteristics were determined as vimentin+; A: Nestin+; b: and cytokeratin-; C: by immunofluorescent staining (upper panel) and CD10+ and CD45- by flow cytometry (lower panel). D-F: are corresponding reagent negative controls for vimentin, Nestin and cytokeratin stainings, respectively. Nuclei were either stained with 7AAD (red) or DAPI (blue). Scale bare: 50 μm
