Figure 3.

Effect of LPS on TLR4 and MyD88 expression in ESCs. ESCs were stimulated with different concentrations of LPS for 8 hr or with 100 ng/ml at different time intervals (2-24 hr) and the expression of TLR4; A, B: and MyD88 transcripts; C, D: was then assessed. Control wells received vehicle. Representative TLR4 and MyD88 PCR bands are shown at the bottom of each graph. E: Effect of LPS activation on TLR4 protein expression by ESCs was evaluated by Western blotting. ESCs1-3: Endometrial stromal cells from three representative participants. AU: Arbitrary unit, *: p < 0.05