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. 2004 Jul;186(14):4714–4729. doi: 10.1128/JB.186.14.4714-4729.2004

FIG. 2.

FIG. 2.

Operon mapping by RT-PCR analysis of iron- and Fur-regulated genes. The template RNA was purified from mid-log-phase bacteria grown in iron-rich and iron-limited MEM-α for iron-induced (exbB3, exbD3, and Cj0111) and iron-repressed genes, respectively. Predicted RT-PCR fragments with gene names are shown at the bottom. The gel lanes match the RT-PCR fragment labels. Lanes M1 and M2 show the 1-kb and 100-bp DNA ladders, respectively.