TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli DH5α | endA1 hsdR17(rK− mK−) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA argF U169 deoR [φ80dlacΔ(lacZΔM15)] | Invitrogen |
| C. jejuni | ||
| AS 144 | C. jejuni NCTC 11168 | National Collection of Type Cultures |
| AS 230 | AS 144 Δfur | This study |
| AS 269 | AS 144 ΔcfrA | This study |
| AS 265 | AS 144 ΔceuE | This study |
| AS 211 | AS 144 ΔCj0178 | This study |
| Plasmids | ||
| pUC19 | Cloning and suicide vector, Ampr | Biolabs |
| pRY 111 | Cmr resistance gene | 57 |
| pAS 226 | pUC19 carrying fur | This study |
| pAS 227 | pUC19 carrying Δfur | This study |
| pAS 229 | pUC19 carrying Δfur::cmr | This study |
| pAS 266 | pUC19 carrying cfrA | This study |
| pAS 267 | pUC19 carrying ΔcfrA | This study |
| pAS 268 | pUC19 carrying ΔcfrA::cmr | This study |
| pAS 261 | pUC19 carrying ceuE | This study |
| pAS 262 | pUC19 carrying ΔceuE | This study |
| pAS 263 | pUC19 carrying ΔceuE::cmr | This study |
| pAS 207 | pUC19 carrying Cj0178 | This study |
| pAS 208 | pUC19 carrying ΔCj0178 | This study |
| pAS 209 | pUC19 carrying ΔCj0178::cmr | This study |
a
Cmr, chloramphenicol resistance gene; Ampr, ampicillin resistant.