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. 2004 Jul;186(14):4492–4501. doi: 10.1128/JB.186.14.4492-4501.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 5. — Controlled expression of phoB influences biofilm formation at high and low phosphate concentrations. Dose-response curves of A. tumefaciens TD5 (phoB::pTD105) harboring pTD115 (P_traI::phoB) over a range of AAI concentrations after 48 h of growth at 50 (A) or 500 (B) μM phosphate are shown. In panels A and B, the OD₆₀₀ of planktonic cells (filled circles), the A₆₀₀ values of DMSO-solubilized CV from adherent cells (filled squares), and the CV A₆₀₀/OD₆₀₀ ratio (filled triangles) are shown. Dashed lines with open symbols correspond to filled symbols but are for A. tumefaciens NTL4 (wild-type phoB) without pTD115. (C) AP activities in response to AAI dose at 50 (inverted triangles) and 500 (upright triangles) μM phosphate in either A. tumefaciens TD5(pTD115) (filled symbols) or A. tumefaciens NTL4 without pTD115 (open symbols).